Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
Features
Thermostable—half life is more than 40 min at 95°C
Generates PCR products with 3'-dA overhangs
Supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)
Applications
Routine PCR amplification of DNA fragments up to 5 kb
High throughput PCR
DNA labeling
Notes
The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
10X Taq Buffer without Detergent is recommended for microarray experiments.
10X Taq Buffer with (NH4)2SO4 allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.