Taq PCR Master Mix contains Taq DNA Polymerase, the unique QIAGEN PCR Buffer that minimizes the requirement for optimization, and dNTPs. The convenient 2x master mix format reduces pipetting steps, increasing throughput and reproducibility, while reducing the risk of contamination.
Performance:
TheTaq PCR Master Mix Kit outperformed kits tested from other suppliers and ensures reliable PCR performance in a wide range of PCR applications — without the need for time-consuming optimization. Taq DNA Polymerase ensures highly specific amplification with different primer–template systems. Every lot of QIAGEN's Taq DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified from human genomic DNA. Due to the unique PCR buffer included in the master mix, optimization of PCR by varying the annealing temperature or the Mg2+ concentration is dramatically reduced and often not required.
Specification:
Concentration: 5 units/µl
Recombinant enzyme: Yes
Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTP
Extension rate: 2–4 kb/min at 72°C
Half-life: 10 min at 97°C; 60 min at 94°C
Amplification efficiency: ≥105 fold
5'–>3' exonuclease activity: Yes
Extra A addition: Yes
3'–>5' exonuclease activity: No
Contaminating nucleases: No
Contaminating RNases: No
Contaminating proteases: No
Self-priming activity: No