TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, TrypLE Express is:
• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)
This TrypLE Express is modified as follows:
With EDTA
Without Phenol Red