Zero Blunt® TOPO® PCR Cloning provides a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of blunt-end PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Each kit has the Zero Blunt™ TOPO™ vector containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants. Zero™ Blunt™ TOPO™ kits are available with a variety of competent cells, or without competent cells, depending on your needs and budget. Features of the Zero™ Blunt™ TOPO™ PCR Cloning Kits:
• Fast and easy—go from PCR to clones in just 3 steps and in as little as 5 minutes hands-on time
• Efficient—obtain up to 95% clones with correct insert
• Proven—reliable performance for over a decade with over 4,000 citations
• Simple —no ligase, post-PCR procedures, or PCR primers containing specific sequences are required.