11 fragments, from 100bp to 1,000bp in 100bp increments with additional band at 1,500bp. The 500bp fragment is present at increased intensity for easy identification. Recommended Loading: Load 5μl/lane. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase, allowing visualization by autoradiography.