For efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5minutes (blunt-ended inserts in as little as 15minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.