Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.
Highlights:
- High fidelity (25X Taq)
- Fast PCR due to short extension times (15–30 s/kb)
- Robust performance, minimal optimization needed
Applications:
- Amplification of difficult (GC-rich) templates
- High-fidelity PCR
- Cloning
- Template generation for sequencing
- Long-range PCR (up to 20 kb)
- Mutagenesis
- High throughput PCR