The ATLab @ IMCB is providing human induced pluripotent stem cell (iPSC) derivation service. These hiPSCs retain the same genetic makeup of the human donor and can be differentiated into a variety of desired human cells.
1. Reprogramming human somatic cells (e.g. skin fibroblasts or PBMCs) using Sendai virus method
Customer needs to have an approved IRB prior to commencement of service
Service takes 2 – 3
months to complete
Cells will be verified to be mycoplasma-free prior to handing over to customer
iPSCs will be handed over to customer in a 6wp as live cells (feeder-free; 1 cell line per well)
2 frozen vials per line will also be provided
All human primary cells and iPSCs generated will be returned to customer upon completion of order
2. Characterisation services (customized service; charges to be determined separately after consultation)
A. IHC for pluripotency markers: OCT4, SOX2, NANOG, SSEA4 and TRA-1-60; nuclear staining: DAPI. Scale bar 50 μm
B. Karyogram of human somatic cell-derived iPSC.
C. Haematoxylin and eosin staining of in vivo-derived teratoma sections from iPSC.
Left to right: intestinal-like epithelium (Definitive Endoderm); cartilage (Mesoderm) and neuronal rosette (Ectoderm).