Next Level Genomics Pte Ltd

Next Level Genomics (NLG) offers cutting-edge spatial genomics and long-read sequencing services, supporting you at every stage—from sample preparation to comprehensive data analysis. With state-of-the-art technology and expert guidance, we provide tailored solutions to drive your research forward.

What we offer

State-of-the-art technology

NLG is the first in Singapore to offer the PacBio Revio system and also utilizes the Oxford Nanopore PromethION-24 and NanoString's GeoMx and CosMx platforms, giving researchers access to the latest in genomics research tools.

End-to-end support

NLG offers a full suite of services, from sample preparation and quality control to data analysis, ensuring seamless integration and high-quality results.

Tailored solutions

NLG customizes its services to meet the specific needs of clients in academia, biotech, and pharma, helping to unlock novel biomarkers and therapeutic targets.

Key Services

Spatial Genomics

NanoString GeoMx DSP (Digital Spatial Profiling): For spatial analysis of protein and RNA expression.
NanoString CosMx SMI (Spatial Molecular Imaging): For high-resolution, multiplexed imaging of RNA and proteins within tissue samples at single cell resolution.

Long Read Sequencing

PacBio Revio and Sequel IIe Systems: For high-accuracy long-read sequencing supporting whole-genome, RNA, single-cell RNA, microbiome, and targeted sequencing.
Oxford Nanopore PromethION-24: Provides real-time, long-read sequencing for de novo genome assembly, structural variant detection, and transcriptomic analysis.

Comprehensive End to End Support

Sample and Slide Preparation: Expertise in preparing a wide range of sample types for both spatial and sequencing workflows.
Data Analysis: Cutting-edge bioinformatics services, including data interpretation and customized reports, ensuring actionable insights
Quality Control and SOP Development: NLG ensures high-quality standards and optimal performance through stringent QC and development of tailored standard operating procedures (SOPs).

Applications

Cancer Research

Tumor Microenvironment Analysis: Using spatial genomics to study gene and protein expression within tumor tissues, helping to identify cancer-driving mechanisms and therapeutic targets.
Biomarker Discovery: Uncover novel biomarkers for cancer diagnosis, prognosis, and therapy response prediction.

Disease Progression and Therapeutic Response

Tracking Disease Evolution: Long-read sequencing allows for comprehensive profiling of disease-associated genetic mutations, structural variants, and transcriptomic changes over time.
Spatial Atlas Development (SpACE-Dx): Mapping the clinical evolution of diseases, such as cancer, to identify new therapeutic targets.

Single-Cell and Transcriptomic Analysis

RNA Sequencing: High-resolution RNA sequencing for in-depth analysis of gene expression at the single-cell or population level, enabling discoveries in developmental biology and immune responses.

Microbiome Research

Microbial Genome Assembly: Long-read sequencing of microbial communities to study the role of microbiota in health and disease, including gut health, infections, and antimicrobial resistance.

Pharmacogenomics and Drug Development

Therapeutic Target Validation: NLG’s platforms help researchers identify and validate genetic targets for drug development, improving precision medicine strategies.
Response to Therapies: Spatial profiling and sequencing data provide insights into how different patient populations respond to specific therapies.

Genetic Disorder Research

Structural Variant Detection: Long-read sequencing technologies excel at identifying complex structural variants and repeat expansions linked to genetic disorders, providing insights for diagnosis and treatment.

De Novo Genome Assembly

High-Quality Genome Reconstruction: Oxford Nanopore and PacBio platforms allow the assembly of complex genomes, essential for agricultural research, biodiversity studies, and evolutionary biology.

Key Equipment

	PacBio Revio System: NLG is the first in Singapore to offer the PacBio Revio system, providing high-accuracy, long-read sequencing with applications in whole genome, RNA, and targeted sequencing. Enables comprehensive analysis of complex genetic regions, including structural variants and repeat expansions. PacBio Sequel IIe System: Complements the Revio platform by offering versatile long-read sequencing options for a broad range of applications, including microbial genomics and immune repertoire analysis.
Oxford Nanopore PromethION-24 (P24): Installed in 2024, this powerful sequencer allows for 24 independent flow cell sequencing, supporting applications like de novo genome assembly, structural variant detection, and transcriptomic analysis. Offers unmatched flexibility for a wide range of sample types, including complex and mixed populations
	NanoString CosMx Spatial Molecular Imaging (SMI): High-resolution platform enabling in situ visualization of RNA and protein expression at single-cell resolution within the tissue architecture. Supports deep spatial understanding of tissue heterogeneity and disease microenvironments.
NanoString GeoMx Digital Spatial Profiling (DSP): Advanced spatial profiling platform for high-throughput, multiplexed protein and RNA analysis in tissue samples. Facilitates biomarker discovery by revealing spatial relationships within tissues, crucial for cancer and disease research.
NanoString nCounter: A highly sensitive platform for direct, digital multiplexed measurement of nucleic acids and proteins.Used for gene expression profiling, copy number variation analysis, and miRNA profiling, the nCounter is particularly valuable for biomarker discovery, precision oncology, and immune profiling. Allows for high-throughput data generation with minimal sample preparation, making it a robust tool for translational research.

For more information, please contact enquiriies@rsc.a-star.edu.sg or spatial@nextlevelgenomics.com (for Spatial) and david@nextlevelgenomics.com (for Sequencing)