Immunomonitoring Service Platform (ISP)

Singapore Immunology Network (SIgN) hosts a state-of-the-art Immunomonitoring (IM) platform with an integrated suite of capabilities including:

Flow Cytometry
Multiplex Analysis of Proteins (MAP)
Computational Immunology

With applications in immune profiling, biomarker discovery, target identification, vaccine assessment, patient treatment stratification and clinical trial monitoring, the SIgN IM platform has more than 130 successful partnerships and over 1,164 scientific publications since 2007 (as of 2019).

Key Capabilities

Largest flow cytometry platform in South East Asia

ISO 9001:2015 certified
ASCP board-certified staff
Up to 14 analyzers & 8 sorters
Analysis of up to 28 colours

Scope of Services

Panel design
Sample preparation and staining
Cell sorting
Cell analysis
Data analysis

Samples

Human
Mouse
Non-human primates
Porcine
Drosophilia
Yeast

Applications (non-exhaustive)

Immunophenotyping

Cell cycle analysis

Cell proliferation assay

Protein engineering detection

Apoptosis assay

Cytotoxicity

Microorganisms detection

Index sorting

Calcium flux assay

Telomere length assay

Side population

Sensitive detection of cytokines, chemokines & metabolites

High-throughput, simultaneous analysis of up to 65 analytes
Samples volume as low as 25ul

Exploratory panel kits (Immune-related research)

Human Immune monitoring
Chemokine/ Cytokine multi-plex kits
Customisable analytes of interest

Scope of Services

Study design
Measurement of multiple targets using Luminex xMAP ®, ultra-sensitive immunoassay and ELISA
Customization of immunoassays (standard/ exploratory format)
Metabolic profiling using Seahorse technology

Samples

Mouse, human, rat, horse, feline, porcine
Human plasma, serum, urine, tears, breast milk

Applications (non-exhaustive)

Inflammation

T cell activation

Apoptosis

Metabolism

Immune cell profiling

Integration of hetergeneous data sets to accelerate research

Novel analysis tools
Interactive visualisations

Scope of Services

Cross-platform data analysis
Next Gen Seq (NGS) analysis
Flow cytometry analysis
Large scale cohort studies & clinical trials data analysis

Applications (non-exhaustive)

1. Analysis of NGS data which may include:

RNA-Seq
ChIP-Seq
ATAC-Seq
Variant calling
Genome assembly

2. Analysis of hetergeneous data including:

Gene expression data
Genetics data
Metabolomics data
Clinical data
Immunological data