Research Support Centre
Technology Platforms

Immunomonitoring by Singapore Immunology Network (SIgN)

The state-of-the-art Immunomonitoring (IM) platform hosted within the Singapore Immunology Network (SIgN) boasts an integrated suite of capabilities including:
  1. Flow Cytometry*
  2. Multiplex Analysis of Proteins (MAP)*
  3. Computational Immunology*
  4. Spatial Immuno-Phenomics
With applications in immune profiling, biomarker discovery, target identification, vaccine assessment, patient treatment stratification and clinical trial monitoring, the SIgN IM platform has more than 130 successful partnerships and over 1,164 scientific publications since 2007 (as of 2019).


Key Services


(1) Flow Cytometry

A well-established flow cytometry platform, with cutting-edge instrumentatio and trained support staff
  • ISO 9001:2015 certified
  • ASCP board-certified staff
  • 6 analyzers & 5 sorters
  • Panel design and analysis of up to 40+ colours spectrally




Flow Cytometry Analyzers and Sorters includes:

  • Agilent NovoCyte Penteon
  • BC CytoFLEX LX
  • BD FACSymphony A3 (Alto)
  • BD FACSymphony A3 (Tenor)
  • BD FACSymphony A5 SE (Soprano)
  • Cytek Aurora
  • BD FACSAria Fusion (Bach)*
  • BD FACSAria Fusion (Vivaldi)*
  • BD FACSAria Fusion (Chopin)*
  • BD FACSAria III**
  • Invitrogen BigFoot*
* Cell sorter
** Cell sorter for yeast/bacteria


    (2) Multiplex Analysis of Proteins

    High throughput, simultaneous analysis of up to 65 analytes (cytokines, chemokines, growth factors)
    • Multiple targets measurement by Luminex FLEXMAP 3D® analyzer
    • Ultra-sensitive measurements at fg/ml concentration
    • Up to 14 analyzers & 8 sorters
    • Metabolic profiling - Seahorse XF Analyzer




    Multiplex Analysis of Proteins Analyzer includes:

    Multiplex Analyzers

    • Multiplex Analyzer - Luminex® xMAP
    • Seahorse XF Multiplex Analyzer
    • CTL ImmunoSpot Analyzer

    Singleplex Analyzers

    • Simoa® HD-1 Analyzer


    (3) Computational Immunology

    Experienced data integration of heterogeneous and diverse biological data

    • Interactive
    • Multi-modal
    • Reproducible




    Bioinformatics Scope includes:

    1. High-Performance Computing for Next Generation Sequencing (NGS) analysis

    Data analysis of Next Generation Sequencing (NGS) data using in-house developed workflows utilizing best-of-breed computational methods. Quality control steps will be used to assess the data prior to analysis. Analysis of data to transform raw data into meaningful biological results.

    2. Multi-modal analysis of biological data

    Analysis of data from multiple heterogeneous sources to identify patterns and associations in the data.

    3. Analysis for Single-cell RNAseq (scRNA-seq), Multiplex Immunohistochemistry & Mass/Flow Cytometry Data Analysis



    10x Genomics Chromium instrument is also available to provide:

    • 10x Genomics gene expression profiling
    • 10x Genomics immune profiling (human/mouse)