Immunomonitoring by Singapore Immunology Network (SIgN)

The state-of-the-art Immunomonitoring (IM) platform hosted within the Singapore Immunology Network (SIgN) boasts an integrated suite of capabilities including:

Flow Cytometry*
Multiplex Analysis of Proteins (MAP)*
Computational Immunology*
Spatial Immuno-Phenomics

With applications in immune profiling, biomarker discovery, target identification, vaccine assessment, patient treatment stratification and clinical trial monitoring, the SIgN IM platform has more than 130 successful partnerships and over 1,164 scientific publications since 2007 (as of 2019).

Key Services

(1) Flow Cytometry

A well-established flow cytometry platform, with cutting-edge instrumentatio and trained support staff

ISO 9001:2015 certified
ASCP board-certified staff
6 analyzers & 5 sorters
Panel design and analysis of up to 40+ colours spectrally

Flow Cytometry Analyzers and Sorters includes:

Agilent NovoCyte Penteon
BC CytoFLEX LX
BD FACSymphony A3 (Alto)
BD FACSymphony A3 (Tenor)
BD FACSymphony A5 SE (Soprano)
Cytek Aurora
BD FACSAria Fusion (Bach)*
BD FACSAria Fusion (Vivaldi)*
BD FACSAria Fusion (Chopin)*
BD FACSAria III**
Invitrogen BigFoot*

* Cell sorter

** Cell sorter for yeast/bacteria

(2) Multiplex Analysis of Proteins

High throughput, simultaneous analysis of up to 65 analytes (cytokines, chemokines, growth factors)

Multiple targets measurement by Luminex FLEXMAP 3D® analyzer
Ultra-sensitive measurements at fg/ml concentration
Up to 14 analyzers & 8 sorters
Metabolic profiling - Seahorse XF Analyzer

Multiplex Analysis of Proteins Analyzer includes:

Multiplex Analyzers

Multiplex Analyzer - Luminex® xMAP
Seahorse XF Multiplex Analyzer
CTL ImmunoSpot Analyzer

Singleplex Analyzers

Simoa® HD-1 Analyzer

(3) Computational Immunology

Experienced data integration of heterogeneous and diverse biological data
Interactive
Multi-modal
Reproducible

Bioinformatics Scope includes:

1. High-Performance Computing for Next Generation Sequencing (NGS) analysis

Data analysis of Next Generation Sequencing (NGS) data using in-house developed workflows utilizing best-of-breed computational methods. Quality control steps will be used to assess the data prior to analysis. Analysis of data to transform raw data into meaningful biological results.

2. Multi-modal analysis of biological data

Analysis of data from multiple heterogeneous sources to identify patterns and associations in the data.

3. Analysis for Single-cell RNAseq (scRNA-seq), Multiplex Immunohistochemistry & Mass/Flow Cytometry Data Analysis

Immunogenomics
10x Genomics Chromium instrument is also available to provide:
10x Genomics gene expression profiling
10x Genomics immune profiling (human/mouse)