IMCB Flow Cytometry Core Facility
Flow Cytometry is an established and essential platform in any research setting. It can be used for a wide range of applications and spans across different research interests.
The IMCB Flow Cytometry Core Facility provides researchers with cell sorting and analysis services. While the cell sorter is strictly operated by our trained and dedicated staff, users will be able to operate the analysers independently once they have completed adequate training sessions.
The Flow Cytometry Core Facility is directed by Dr Anna-Marie Fairhurst and managed by Mr Daniel Anand Silva.
Flow cytometry is a laser-based technology typically used to measure fluorescence intensity or scattered light, to obtain valuable information of the cells by analyzing their physical properties.
Flow cytometry has become readily available in research and clinical settings, and its applications have advanced rapidly in parallel with significant improvements in instrumentation and the availability of a wide array of monoclonal reagents. Properly performed, flow cytometry can provide rapid and accurate characterization of your cells of interest.
We provide sorting and analysis services across a wide range of applications and research areas.
Please find in the main page our equipment configuration with the list of the possible fluorochromes that you can use. To facilitate planning of your experiment you can consult the BD Spectral Viewer Tool or other panel design tools online.
If you wish to plan a cell sort, kindly adhere to IMCB safety regulations. To book for a sort, please download and fill up the facilty booking form and send your request to IMCB Flow Cytometry, or call 6586 9908.
If you are a first time user, please contact our staff at IMCB Flow Cytometry to arrange for an initial training session if necessary. Once you have completed the training, you may operate the instrument independently
BD Arial II (4 Lasers) - Sorter
|BD FACSSymphony (6 Lasers) - Analyzer|
|BD LSRII (4 Lasers)|
|Cytek Aurora (3 Lasers)|
Facility Director – Dr Anna-Marie Fairhurst
Anna-Marie Fairhurst received her PhD in Immunology/Pharmacology at the William Harvey Research Institute at St Bartholomew’s Hospital, University of London in 2002. During her PhD she examined the roles of neutrophil-FcγRIIIb and tristetraprolin (TTP) expression in rheumatoid arthritis under the direction of Nicolas Goulding and Paul Wallace (Dartmouth, NH).
From London she moved to the NIH, in Bethesda, Maryland, USA to undertake a postdoc with Peter Lipsky. Projects were focused on the mechanisms of the initiation and progression of autoimmunity. Her main directions were to examine innate regulation of systemic lupus erythematosus (SLE) and the effects of IL-21 on B cell transcription factors.
In 2004, she moved to Dallas, TX, to continue studying SLE with Ward Wakeland. Her research focused on the genetic and immunological mechanisms which drive SLE pathogenesis using multiple murine models, specifically examining the role of IFNα and the TLRs.
Anna-Marie joined A*STAR in March 2010 at the Singapore Immunology Network and transferred to IMCB in 2019. The overall goal of her lab is to understand the mechanisms that maintain an effective balance in the immune system for efficient protection from infections, cancer and autoimmunity. Systemic lupus erythematosus (SLE) is the archetypal autoimmune disease, whereby the host’s immune system develops reactivity to self, resulting in systemic inflammation and often, kidney pathology.
By studying the immunological mechanisms driving this disease, the crucial mechanisms of immune tolerance and inflammation that are targets for therapy across multiple diseases can be understood. Her lab is translational, working with multiple animal models and clinical human samples.
She has also retained a passion for advanced flow cytometry techniques and advancing women in STEM. In her spare time she enjoys running, swimming and chasing after her 3 young children.
Facility Assistant Director – Daniel Anand Silva
Daniel has extensive flow cytometry experience, having worked in the field since 2014. He started in the BSF Scientific Services shared facility in Jan 2014, working as an application specialist for them till Oct 2015. He then moved over to SigN in October 2015 to run the BMSI FACS services for them till Jan 2018. Daniel led a team of 2-3 pax in running sorting and analysis services for a wide range of applications for the wider A-STAR scientific community and external clients.
He then moved to Covance as a medical technologist in February 2018. Worked in the hematology department for 2 months, doing workflow optimization for clinical sample processing. He then moved to Duke-NUS Graduate Medical School in May 2018 as the Flow Facility manager and worked there till September 2019, before starting at IMCB.
Daniel is passionate about technology and the endless possibilities that it provides the research community. He enjoys educating users and researchers about flow cytometry and consulting on experiment planning and optimization.
In his spare time, Daniel enjoys engaging in sports and reading. He is constantly looking to improve himself and would be glad to assist you on your flow projects.
For more information, please contact firstname.lastname@example.org or email@example.com
1. Access to IMCB Flow Cytometry's PPMS online booking system