High Throughput Phenomics (HTP) by EARO@EDDC

Quantify the Most Subtle Changes in Cellular Morphology

The High Throughput Phenomics (HTP) platform specializes in image-based assay and caters to drug discovery projects involving cell-based assays. It combines automated imaging and quantitative data analysis in a high-throughput format suitable for large-scale applications such as drug discovery and systems biology. Several focused and diversity compound libraries of over 300,000 curated compounds are available for screening.

HTP also supports access to many of the listed high throughput instruments for independent operation by external users on a pay-per-use basis.

Scope of Services

Image-based Drug Screening

Biochemistry-based Drug Screening

Target & Hit Identification

Assay Optimsation & Miniaturisation
- Cell-based Assays
  (Proliferation, Cell Signalling, Protein Interaction, etc.)
- Biochemical Assays
  (AlphaLISA, Fluorescence Polarization, etc.)

Key Image-based Techniques

Cell Painting

3D Spheroid Characterisation

Heterogeneous Co-culture Modelling

Cellular Activity
(Migration, Outgrowth Localisation, Phosphorylation, etc.)

Sample Types

Cultured Mammalian Cells

Spheroid/Organoid Cultures

Whole-mount Tissue Sections

Key Instruments/Technologies

High Content Imaging Systems
- Perkin Elmer Opera Phenix
- Perkin Elmer Operetta CLS

High Throughput Liquid Handling Systems
- Agilent Bravo Integrated Deck
- Perkin Elmer Janus Integrated Deck
- Beckman-LabCyte Echo 550

Microplate Washers & Dispenser
- Agilent-BioTek 405TS Washer with Stacker
- Agilent-BioTek MultiFlo FX with Random Access Dispenser
- Agilent-BioTek MultiFlo FX Bulk Dispenser
- Thermo Multidrop Combi Bulk Dispenser

High Throughput Plate Readers
- Tecan Spark Multimode Reader
- Li-Cor Odyssey SA Infrared Scanner

Multispectral Whole Slide Scanner
- Akoya Vectra Polaris/Phenolmager HT

Flow Cytometers
- Luminex Amnis ImageStreamX mkII Imaging Flow Cytometer
- Beckman CytoFLEX SRT Cell Sorter

To learn more about EARO, please visit www.earo.sg.

The HTP Platform is part of the EARO initiative, whose mission is to accelerate early-stage drug discovery in Singapore by providing access to EDDC’s expertise in Drug Discovery & Development and its industry-leading technologies.